Composite

Part:BBa_K5250012:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Pxut + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 822
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 210
    Illegal AgeI site found at 671
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences have been codon optimized.


Source

The Pxut sequence we obtained from the following paper: https://doi.org/10.1128/aem.00250-20. The sequence of the GFP is from the iGEM Parts Registry.

References